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Image Search Results
Journal: Cancers
Article Title: Autocrine Signaling of NRP1 Ligand Galectin-1 Elicits Resistance to BRAF-Targeted Therapy in Melanoma Cells
doi: 10.3390/cancers12082218
Figure Lengend Snippet: Galectin-1 upregulation in melanoma samples and cell lines resistant to BRAF-targeted therapy. ( A ) The graph shows Galectin-1 (LGALS1 gene) mRNA expression variation in paired melanoma samples (obtained from seven patients, indicated by black diamonds) before treatment with BRAF inhibitors and after the onset of secondary resistance (Log2 ratio resistant/pretherapy), as well as in A375 and SK-MEL-28 melanoma cells before and after developing resistance to 2μM PLX-4720 (Log2 ratio Resistant/Parental; n = 3). ( B ) Analysis of Galectin-1 protein levels (by PikoKine ELISA assay) in conditioned media (CM) derived from either parental or PLX4720-resistant A375 and SK-MEL-28 (PLX-resistant) cells. Data are normalized to levels detected in the respective parental cells and represent the average of a technical duplicate. Statistical significance was assessed by t -test, comparing parental and their derived PLX-resistant cell lines; *** p < 0.0001.
Article Snippet: Galectin-1 concentrations in CM were measured by
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay